Clinical and bacteriologic correlates of the PapG alleles among uropathogenic escherichia coli strains isolated from cases of adult urinary tract infection

To study the distribution of papG gene in uropathogenic Escherichia coli [E.coli] strains isolated from adult urinary tract infection [UTI] and the relationship between the different classes of papG gene and patients, sex, hospitalization and their clinical forms of UTI. Laboratory study. Inpatient and outpatient settings with laboratory investigation. Genotyping of papG, the adhesion gene of E. coli P fimbriae, may predict clinical outcomes of UTI. A total of 182 urinary E .coli strains were analyzed by multiplex PCR method for detection of papG gene. Patients, sex, hospitalization and their clinical forms of UTI were also evaluated. The distribution of papG gene in uropathogenic E.coli strains and the relationship between papG gene and clinical features of the patients. Multiplex PCR method was performed for detection of papG gene in uropathogenic E.coli strains isolated from adult urinary tract infections The prevalence of pap operon in the uropathogenic isolates was 36.2%. The prevalence of papG gene classes II and III in uropathogenic isolates was 23.1% and 6.6% respectively. None of the isolates had class I genotype. PapG classes II and III were predominant in patients with pyelonephritis and cystitis respectively. There was no significant relationship between the presence of papG alleles, sex and hospitalization of the patients. PapG gene is likely to play an important role in pathogenesis of uropathogenic strains of E.coli in adult nosocomial UTIs. Detection and genotyping of this gene may contribute to improving the management of UTI

Expression of NC-2 receptor on MCL cells and its natural cytotoxicity against cancer cells

To identify the expression of NC-2 on an interleukin-3 dependent mast cell line [MCL] and investigate the activity of this receptor against tumor cells. Laboratory study. Cellular and Molecular Center, Shahrekord, Iran. The MCL cells were stained with D9 monoclonal antibody [anti-NC-2] and analysed by flowcytometry. This was confirmed by immunoperoxidase staining. The cytotoxicity assay was performed to show the cytotoxic activity of MCL cells against 51Cr-labelled WEHI-164 tumor cells. The expression of NC-2 on MCL cells, and the anti-tumor activity of this receptor were investigated. Flow cytometric analysis and in vitro experiments were performed for showing the activity of NC-2 against cancer. NC-2 receptor was expressed on more than 95% of MCL cells. Pretreatment with D9 monoclonal antibody resulted in about 63% reduction in natural cytotoxicity of MCL cells against WEHI-164 tumor target cells. NC-2 is also one of the receptors expressed on MCL and utilized for WEHI-164 tumor cell killing

Prevalence of asymptomatic bacteriuria and associated host factors in women with type 2 diabetes in shahre-kord, iran; 2005

Asymptomatic bacteriuria [ASB] is common among diabetic women. The aim of this study was to determine the prevalence and risk factors for ASB in women with diabetes. Prospective cross-sectional study. Chahar-Mahal province, Iran One hundred women with type 2 diabetes and 100 normal women [control] who had no abnormalities of the urinary tract were recruited. Demographic data and information regarding previous history of urinary tract infections [UTIs], duration of diabetes and presence of pyuria was collected. Baseline serum creatinine and fasting blood sugar levels [FBSL] were measured. Prevalence and risk factors for ASB in normal and type 2 diabetic women. The prevalence of ASB and related risk factors in the two groups and the rate of ASB progressing to symptomatic UTI in a six-month period were evaluated. The prevalence of ASB was 20% in diabetics and 4% in control group [p < 0.05]. Pyuria was present in 80% of diabetics with ASB. Symptomatic UTI in previous year was the only risk factor for ASB [p < 0.05]. During a follow-up of six months, 40% diabetics with ASB developed to symptomatic UTI. The prevalence of ASB is increased in women with diabetes. We recommend screening for detection and treatment of ASB in diabetic women

Comparison of an [HM -CAPs] ELISA test and the gold standard tests for detecting helicobacter pylori infection

Helicobacter pylori [H. pylori] is one of the main causes of gastritis and is highly related to gastric and duodenal cancer. Different methods are i n t roduced for diagnosis of bacterial infections, but serologic tests are more common. In this study, an ELISA [Enzyme linked immunosorbent assay] test was performed using bacterial high molecular cell-associated proteins [HM-CAPs] containing urease. The sensitivity and specificity of this method was then compared with those using histological [Giemsa and/or modified Gram staining] as well as bacterial culture findings, both considered as gold standard tests for diagnosis. HM-CAPs containing bacterialurease, were extracted by NOG [n-octyl-beta-Dglycopyranoside] detergent and purified on sephacryl gel S-300 HR. Besides, biopsy specimens from antral portion of gastric mucosa together with sera of 168 patients [85 male and 83 female], suspected of H. pylori infection, were obtained. The results of culture and/or histological examinations [gold standard tests] were compared with those of serum ELISA test. Sensitivity and specificity of HM-CAPs ELISA test as compared with histological findings and/or bacterial culture were 93.3% and 95.1% respectively. ELISA test using HM-CAPs antigen is highly specific and sensitive. Therefore, it could be used for serological diagnosis of H. pylori infection

[Bacteriologic study of 50 patients with deep neck infections, Shahrekord, 2001]

Background and aim: deep neck infections are relatively common infections with severe mortality and morbidity outcome. The aim of this study was to determine the type of causing bacteria and their sensitivity pattern to routine antibiotics

Methods: samples were obtained by aspiration from the neck abscesses of 50 patients with deep neck infection referred to Shahrekord Kashani and Resalat hospitals. The samples were then cultured in aerobic and anaerobic media

Results: from 66 bacterial isolates, 35 cases [54%] were identified as strict and 31 cases [46%] were facultative anaerobic bacteria. No strict aerobic bacteria were isolated. Bacteroides and Fusobacterium spp. were the most common strict and coagulase negative staphylococci were the most common facultative anaerobic isolates. In five cases we were not able to identify the causative organisms. In antibiogram testing, cloxacillin, ceftizoxime and chloramphenicol were the most effective antibiotics on the anaerobic bacteria

Conclusion: with respect to fatal nature of deep neck infections, rapid diagnosis and treatment play a main role in reducing the mortality and morbidity of the disease. Ceftizoxime, chloramphenicol and clindamycine antibiotics are recomended for therapeutic purposes of these patients

Bacteriology and sensitivity patterns of acute adult bacterial meningitis: a seven-year overview

Bacterial meningitis is a potentially fatal infection and its rapid diagnosis has an important role in the prognosis of the disease. The objective of this study was to determine the microscopic, bacteriologic and clinical characteristics of adult bacterial meningitis in Chaharmahal province of Iran over a period of seven years. Materials and Thirty-one patients with bacterial meningitis in Shahrekord Kashani Hospital were observed in a period of seven years [Oct. 1993 - Sept. 2000]. The clinical findings, type of bacteria isolated from cerebrospinal fluid [CSF] samples, CSF leucocyte counts, CSF cultures and the antibiotic sensitivity profile of the common bacterial isolates were evaluated. The annual incidence of the disease was about 0.64 per 100,000 inhabitants. Neck stiffness and impairment of consciousness were seen only in a half of Behnam Zamanzad, Hedayatollah Shirzadeh, Mohammad Reza Naficy Department of Microbiology and Immunology, Shahrekord University of Medical Sciences, Shahrekord, Iran the cases. In 50% of patients, the CSF leucocyte counts were 1000-10,000/mm3, while in 26% of the cases the count was below 500/mm3. Eighty-three% of CSF cultures were reported positive. Steptococcus pneumoniae [58%], Neisseria meningitidis [19%] and Gram negative enteric bacilli [6%] were the most common isolates. Gram's stain of sedimented CSF helped in the presumptive identification of the causative bacteria in 80% of the cases. Fifty% of the pneumococcal isolates were resistant to penicillin and 13% were resistant to chloramphenicol. Conclusions: A combination of CSF culture and microscopic examination is highly specific for early diagnosis of bacterial meningitis, and performing an antibacterial sensitivity test is recommended before any antibiotic therapy